Key Skills

Animals

UK Home Office personal license (2016), breeding and maintenance of mutant rodent colonies, managing long-term immunosuppression.

​Stereotaxic surgery - GRIN lens/optic fiber/cranial window implantation, viral injections, cell transplantation

Drug administration via subcutaneous and intraperitoneal injection

Transcardial perfusion, brain microdissection, cardiac puncture, embryo dissection

Oestrus swabbing

Food/water restriction

Head fixed behavioural tasks

Free moving tethered behavioural tasks

Wide battery of motoric, cognitive, sensory, emotional and drug induced behaviours.​

Wet laboratory

Immunohistochemistry - fluorescence, DAB, immunocytochemistry

Tissue sectioning - microtome, cryostat

Microscopy - 2 photon, fluorescence, bright field, time lapse, confocal

PCR, RT-qPCR, western blot, in situ hybridisation, genotyping, primer design, DNA/RNA extraction, CRISPR, transfection, cloning,

PBMC isolation

Cell culture - Human (iPSC, neural derivation, motility assays), mouse (ES, cell line, primary neuronal culture).

Software

Phenosys, Campden Instruments, Med-pc, SPSS Statistics, Graphpad Prism, Matlab, FIJI, Photoshop, Microsoft Office programs